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trmt6 polyclonal antibody  (Proteintech)


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    Proteintech trmt6 polyclonal antibody
    siRNA decreases the mRNA and protein levels of m 1 A regulatory enzymes in HeLa cells. (A) Relative expression changes of mRNA for TRMT10C , ALKBH3 , <t>TRMT6</t> , TRMT61A , and TRMT6-61 A were measured by RT-qPCR. The x-axis represents the treatment groups, while the y-axis indicates the mRNA expression changes of m 1 A regulatory enzymes relative to the reference gene (GAPDH). (B) Protein level changes of TRMT10C, ALKBH3, TRMT6, TRMT61A, and TRMT6-61 A were assessed by Western blotting. Protein bands were quantified using ImageJ software. The x-axis represents the treatment groups, and the y-axis shows the changes in m1A regulatory enzymes relative to the reference gene (GAPDH). Each group was conducted with three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001
    Trmt6 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trmt6 polyclonal antibody/product/Proteintech
    Average 93 stars, based on 7 article reviews
    trmt6 polyclonal antibody - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "Quantifying the mRNA epitranscriptome reveals epitranscriptome signatures and roles in cancer"

    Article Title: Quantifying the mRNA epitranscriptome reveals epitranscriptome signatures and roles in cancer

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-025-05805-7

    siRNA decreases the mRNA and protein levels of m 1 A regulatory enzymes in HeLa cells. (A) Relative expression changes of mRNA for TRMT10C , ALKBH3 , TRMT6 , TRMT61A , and TRMT6-61 A were measured by RT-qPCR. The x-axis represents the treatment groups, while the y-axis indicates the mRNA expression changes of m 1 A regulatory enzymes relative to the reference gene (GAPDH). (B) Protein level changes of TRMT10C, ALKBH3, TRMT6, TRMT61A, and TRMT6-61 A were assessed by Western blotting. Protein bands were quantified using ImageJ software. The x-axis represents the treatment groups, and the y-axis shows the changes in m1A regulatory enzymes relative to the reference gene (GAPDH). Each group was conducted with three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: siRNA decreases the mRNA and protein levels of m 1 A regulatory enzymes in HeLa cells. (A) Relative expression changes of mRNA for TRMT10C , ALKBH3 , TRMT6 , TRMT61A , and TRMT6-61 A were measured by RT-qPCR. The x-axis represents the treatment groups, while the y-axis indicates the mRNA expression changes of m 1 A regulatory enzymes relative to the reference gene (GAPDH). (B) Protein level changes of TRMT10C, ALKBH3, TRMT6, TRMT61A, and TRMT6-61 A were assessed by Western blotting. Protein bands were quantified using ImageJ software. The x-axis represents the treatment groups, and the y-axis shows the changes in m1A regulatory enzymes relative to the reference gene (GAPDH). Each group was conducted with three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot, Software

    Effects of m 1 A regulatory enzyme knockdown on cell viability. (A) CCK8 assay evaluating changes in cell proliferation following knockdown of m 1 A regulatory enzymes TRMT10C , ALKBH3 , TRMT6 , TRMT61A , and TRMT6-61 A at 6 h, 24 h, 48 h, and 72 h. The x-axis represents treatment duration, and the y-axis indicates cell viability. Each group consists of three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001. (B) Flow cytometry measurement of apoptosis rates after 72 h of knockdown of m 1 A regulatory enzymes TRMT10C , ALKBH3 , TRMT6 , TRMT61A , and TRMT6-61 A . Cell apoptosis percentages were quantified using GraphPad Prism software; the x-axis represents m 1 A regulatory enzymes, and the y-axis represents the percentage of apoptosis. Each group consists of three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: Effects of m 1 A regulatory enzyme knockdown on cell viability. (A) CCK8 assay evaluating changes in cell proliferation following knockdown of m 1 A regulatory enzymes TRMT10C , ALKBH3 , TRMT6 , TRMT61A , and TRMT6-61 A at 6 h, 24 h, 48 h, and 72 h. The x-axis represents treatment duration, and the y-axis indicates cell viability. Each group consists of three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001. (B) Flow cytometry measurement of apoptosis rates after 72 h of knockdown of m 1 A regulatory enzymes TRMT10C , ALKBH3 , TRMT6 , TRMT61A , and TRMT6-61 A . Cell apoptosis percentages were quantified using GraphPad Prism software; the x-axis represents m 1 A regulatory enzymes, and the y-axis represents the percentage of apoptosis. Each group consists of three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: Knockdown, CCK-8 Assay, Flow Cytometry, Software

    Transcriptomic and proteomic analysis of TRMT6-61 A treated HeLa cells: Transcriptome: (A) Top 20 KEGG enrichment bubble chart. Proteome: (B) Top 25 connectivity protein interaction network diagram. Combined transcriptomic and proteomic analysis: (C) Bar chart of the top 30 KEGG (GSEA) pathways shared across different omics
    Figure Legend Snippet: Transcriptomic and proteomic analysis of TRMT6-61 A treated HeLa cells: Transcriptome: (A) Top 20 KEGG enrichment bubble chart. Proteome: (B) Top 25 connectivity protein interaction network diagram. Combined transcriptomic and proteomic analysis: (C) Bar chart of the top 30 KEGG (GSEA) pathways shared across different omics

    Techniques Used:



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    siRNA decreases the mRNA and protein levels of m 1 A regulatory enzymes in HeLa cells. (A) Relative expression changes of mRNA for TRMT10C , ALKBH3 , <t>TRMT6</t> , TRMT61A , and TRMT6-61 A were measured by RT-qPCR. The x-axis represents the treatment groups, while the y-axis indicates the mRNA expression changes of m 1 A regulatory enzymes relative to the reference gene (GAPDH). (B) Protein level changes of TRMT10C, ALKBH3, TRMT6, TRMT61A, and TRMT6-61 A were assessed by Western blotting. Protein bands were quantified using ImageJ software. The x-axis represents the treatment groups, and the y-axis shows the changes in m1A regulatory enzymes relative to the reference gene (GAPDH). Each group was conducted with three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001
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    siRNA decreases the mRNA and protein levels of m 1 A regulatory enzymes in HeLa cells. (A) Relative expression changes of mRNA for TRMT10C , ALKBH3 , <t>TRMT6</t> , TRMT61A , and TRMT6-61 A were measured by RT-qPCR. The x-axis represents the treatment groups, while the y-axis indicates the mRNA expression changes of m 1 A regulatory enzymes relative to the reference gene (GAPDH). (B) Protein level changes of TRMT10C, ALKBH3, TRMT6, TRMT61A, and TRMT6-61 A were assessed by Western blotting. Protein bands were quantified using ImageJ software. The x-axis represents the treatment groups, and the y-axis shows the changes in m1A regulatory enzymes relative to the reference gene (GAPDH). Each group was conducted with three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001
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    siRNA decreases the mRNA and protein levels of m 1 A regulatory enzymes in HeLa cells. (A) Relative expression changes of mRNA for TRMT10C , ALKBH3 , <t>TRMT6</t> , TRMT61A , and TRMT6-61 A were measured by RT-qPCR. The x-axis represents the treatment groups, while the y-axis indicates the mRNA expression changes of m 1 A regulatory enzymes relative to the reference gene (GAPDH). (B) Protein level changes of TRMT10C, ALKBH3, TRMT6, TRMT61A, and TRMT6-61 A were assessed by Western blotting. Protein bands were quantified using ImageJ software. The x-axis represents the treatment groups, and the y-axis shows the changes in m1A regulatory enzymes relative to the reference gene (GAPDH). Each group was conducted with three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001
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    (A) Western blot showing increased concentrations of glucose in the growth medium led to decreased ALKBH1 expression in HeLa cells; protein levels of the m1A58-transferase <t>Trmt6/Trmt61</t> heterodimer remained mostly unchanged.
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    Image Search Results


    siRNA decreases the mRNA and protein levels of m 1 A regulatory enzymes in HeLa cells. (A) Relative expression changes of mRNA for TRMT10C , ALKBH3 , TRMT6 , TRMT61A , and TRMT6-61 A were measured by RT-qPCR. The x-axis represents the treatment groups, while the y-axis indicates the mRNA expression changes of m 1 A regulatory enzymes relative to the reference gene (GAPDH). (B) Protein level changes of TRMT10C, ALKBH3, TRMT6, TRMT61A, and TRMT6-61 A were assessed by Western blotting. Protein bands were quantified using ImageJ software. The x-axis represents the treatment groups, and the y-axis shows the changes in m1A regulatory enzymes relative to the reference gene (GAPDH). Each group was conducted with three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Quantifying the mRNA epitranscriptome reveals epitranscriptome signatures and roles in cancer

    doi: 10.1007/s00018-025-05805-7

    Figure Lengend Snippet: siRNA decreases the mRNA and protein levels of m 1 A regulatory enzymes in HeLa cells. (A) Relative expression changes of mRNA for TRMT10C , ALKBH3 , TRMT6 , TRMT61A , and TRMT6-61 A were measured by RT-qPCR. The x-axis represents the treatment groups, while the y-axis indicates the mRNA expression changes of m 1 A regulatory enzymes relative to the reference gene (GAPDH). (B) Protein level changes of TRMT10C, ALKBH3, TRMT6, TRMT61A, and TRMT6-61 A were assessed by Western blotting. Protein bands were quantified using ImageJ software. The x-axis represents the treatment groups, and the y-axis shows the changes in m1A regulatory enzymes relative to the reference gene (GAPDH). Each group was conducted with three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001

    Article Snippet: The protein samples were incubated with the corresponding primary antibodies: TRMT6 Polyclonal Antibody (Proteintech), TRMT10C Polyclonal Antibody (Proteintech), ALKBH3 Polyclonal Antibody (Proteintech), and TRMT61A Polyclonal Antibody (ORIGENE), followed by incubation with the respective secondary antibodies: Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (Proteintech) and Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (Proteintech).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Software

    Effects of m 1 A regulatory enzyme knockdown on cell viability. (A) CCK8 assay evaluating changes in cell proliferation following knockdown of m 1 A regulatory enzymes TRMT10C , ALKBH3 , TRMT6 , TRMT61A , and TRMT6-61 A at 6 h, 24 h, 48 h, and 72 h. The x-axis represents treatment duration, and the y-axis indicates cell viability. Each group consists of three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001. (B) Flow cytometry measurement of apoptosis rates after 72 h of knockdown of m 1 A regulatory enzymes TRMT10C , ALKBH3 , TRMT6 , TRMT61A , and TRMT6-61 A . Cell apoptosis percentages were quantified using GraphPad Prism software; the x-axis represents m 1 A regulatory enzymes, and the y-axis represents the percentage of apoptosis. Each group consists of three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Quantifying the mRNA epitranscriptome reveals epitranscriptome signatures and roles in cancer

    doi: 10.1007/s00018-025-05805-7

    Figure Lengend Snippet: Effects of m 1 A regulatory enzyme knockdown on cell viability. (A) CCK8 assay evaluating changes in cell proliferation following knockdown of m 1 A regulatory enzymes TRMT10C , ALKBH3 , TRMT6 , TRMT61A , and TRMT6-61 A at 6 h, 24 h, 48 h, and 72 h. The x-axis represents treatment duration, and the y-axis indicates cell viability. Each group consists of three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001. (B) Flow cytometry measurement of apoptosis rates after 72 h of knockdown of m 1 A regulatory enzymes TRMT10C , ALKBH3 , TRMT6 , TRMT61A , and TRMT6-61 A . Cell apoptosis percentages were quantified using GraphPad Prism software; the x-axis represents m 1 A regulatory enzymes, and the y-axis represents the percentage of apoptosis. Each group consists of three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001

    Article Snippet: The protein samples were incubated with the corresponding primary antibodies: TRMT6 Polyclonal Antibody (Proteintech), TRMT10C Polyclonal Antibody (Proteintech), ALKBH3 Polyclonal Antibody (Proteintech), and TRMT61A Polyclonal Antibody (ORIGENE), followed by incubation with the respective secondary antibodies: Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (Proteintech) and Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (Proteintech).

    Techniques: Knockdown, CCK-8 Assay, Flow Cytometry, Software

    Transcriptomic and proteomic analysis of TRMT6-61 A treated HeLa cells: Transcriptome: (A) Top 20 KEGG enrichment bubble chart. Proteome: (B) Top 25 connectivity protein interaction network diagram. Combined transcriptomic and proteomic analysis: (C) Bar chart of the top 30 KEGG (GSEA) pathways shared across different omics

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Quantifying the mRNA epitranscriptome reveals epitranscriptome signatures and roles in cancer

    doi: 10.1007/s00018-025-05805-7

    Figure Lengend Snippet: Transcriptomic and proteomic analysis of TRMT6-61 A treated HeLa cells: Transcriptome: (A) Top 20 KEGG enrichment bubble chart. Proteome: (B) Top 25 connectivity protein interaction network diagram. Combined transcriptomic and proteomic analysis: (C) Bar chart of the top 30 KEGG (GSEA) pathways shared across different omics

    Article Snippet: The protein samples were incubated with the corresponding primary antibodies: TRMT6 Polyclonal Antibody (Proteintech), TRMT10C Polyclonal Antibody (Proteintech), ALKBH3 Polyclonal Antibody (Proteintech), and TRMT61A Polyclonal Antibody (ORIGENE), followed by incubation with the respective secondary antibodies: Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (Proteintech) and Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (Proteintech).

    Techniques:

    (A) Western blot showing increased concentrations of glucose in the growth medium led to decreased ALKBH1 expression in HeLa cells; protein levels of the m1A58-transferase Trmt6/Trmt61 heterodimer remained mostly unchanged.

    Journal: Cell

    Article Title: ALKBH1-Mediated tRNA Demethylation Regulates Translation

    doi: 10.1016/j.cell.2016.09.038

    Figure Lengend Snippet: (A) Western blot showing increased concentrations of glucose in the growth medium led to decreased ALKBH1 expression in HeLa cells; protein levels of the m1A58-transferase Trmt6/Trmt61 heterodimer remained mostly unchanged.

    Article Snippet: Polyclonal rabbit anti-TRMT6 antibody (Sigma, SAB2107213) and polyclonal rabbit anti-TRMT61 antibody (Sigma SAB2700607) were both used with 1: 400 dilution.

    Techniques: Western Blot, Expressing